Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody, IgG1, Clone: [VIL1/1314], Mouse, Monoclonal

Product Overview

• Article Name: Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody, IgG1, Clone: [VIL1/1314], Mouse, Monoclonal
• Biozol Catalog Number: NBT-7429-MSM1-P1ABX
• Supplier Catalog Number: 7429-MSM1-P1ABX
• Alternative Catalog Number: NBT-7429-MSM1-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)
• Alternative Names: VIL1, Villin-1, Villin1

Product Description

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin

Product Properties

• Clonality: Monoclonal
• Clone Designation: [VIL1/1314]
• Molecular Weight: 93kDa
• Isotype: IgG1
• NCBI: 7429
• UniProt: P09327
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Western Blot (1-2ug/ml for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed b

Images

Western Blot of A549, HepG2 & HCT116 cell lysates with Villin Monoclonal Antibody (VIL1/1314).

Formalin-fixed, paraffin-embedded human Rectum stained with Villin Monoclonal Antibody (VIL1/1314).

Formalin-fixed, paraffin-embedded human Colon stained with