E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody, IgG1, Clone: [4A2], Mouse, Monoclonal

Product Overview

• Article Name: E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) Antibody, IgG1, Clone: [4A2], Mouse, Monoclonal
• Biozol Catalog Number: NBT-999-MSM4-P1ABX
• Supplier Catalog Number: 999-MSM4-P1ABX
• Alternative Catalog Number: NBT-999-MSM4-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant human E-Cadherin protein
• Alternative Names: Arc 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD324, CDHE, E-Cad/CTF3, ECAD, Epithelial cadherin, epithelial calcium dependent adhesion protein, Liver cell adhesion molecule (LCAM), Uvomorulin (UVO)

Product Description

Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadh

Product Properties

• Clonality: Monoclonal
• Clone Designation: [4A2]
• Molecular Weight: 120-80kDa (Mature), 135kDa (Precursor)
• Isotype: IgG1
• NCBI: 999
• UniProt: P12830
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m

Images

Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Mouse Monoclonal Antibody (4A2).

Immunofluorescence analysis of MeOH-fixed MCF-7 cells. E-Ca