JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
Clonality:
Monoclonal
Clone Designation:
[JC1]
Molecular Weight:
212kDa and 123kDa
Isotype:
IgG2a
NCBI:
Not Known
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followe
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