It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is e
Clonality:
Monoclonal
Clone Designation:
[SPM375]
Molecular Weight:
244Da
Isotype:
IgG1
NCBI:
Not Applicable
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Confocal Microscopy, Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for the
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