MAb 113-1 recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pat
Clonality:
Monoclonal
Clone Designation:
[SPM198]
Molecular Weight:
60kDa
Isotype:
IgG1
NCBI:
Not Known
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunofluorescence (1-2ug/ml), Western Blot (0.25-0.5ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 45 min at 95&
Western Blot Analysis of HeLa cell lysate using Mitochondria Mouse Monoclonal Antibody (SPM198).
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