CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the H
Conjugation:
FITC/RPE
Alternative Names:
CD3 FITC - CD28 PE
The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is expressed on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000
Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Purity:
Protein A/G Chromatography
Formula:
Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Application Notes:
PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gentl
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