CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice im
Conjugation:
FITC/RPE
Alternative Names:
CD4 FITC - CD28 PE
Identification of CD4 on human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. Anti-human CD28 binds the 44kDa MW cell surface protein on the surface of most T cells.
Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Purity:
Protein A/G Chromatography
Formula:
Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Application Notes:
PBMC: Add10 µl of MAB/10 6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gentl
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