Phosphate buffered saline, pH 7.2 with 0.075% sodium azide
Source:
Chickens were immunized with highly purified Toxin A purified from Clostridium difficile. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin A was immunoaffinity purified using the
Purity:
Egg-yolk derived IgY, affinity purified.
Formula:
Phosphate buffered saline, pH 7.2 with 0.075% sodium azide
Application Notes:
This antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:4000 dilution of the IgY was an effective probe for 1 ug of Toxin A. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin A. Optimal
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