Egg-yolk derived affinity purified IgY in phosphate buffered saline, pH 7.2 with 0.075% sodium azide
Source:
Chickens were immunized with highly purified Toxin B purified from Clostridium difficile. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin B was immunoaffinity purified using the
Application Notes:
This antibody is suitable for ELISA and Western Blotting procedures. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin B. This antibody has also been successfully used in cytotoxicity and neutralization assays (please se
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