Mouse anti E-Cadherin / Cadherin-1, IgG1, Clone: [5H9], Monoclonal

Product Overview

• Article Name: Mouse anti E-Cadherin / Cadherin-1, IgG1, Clone: [5H9], Monoclonal
• Biozol Catalog Number: NMB-MUB0301P
• Supplier Catalog Number: MUB0301P
• Alternative Catalog Number: NMB-MUB0301P
• Manufacturer: NordicMubio
• Host: Mouse
• Category: Antikörper
• Application: ICC, IHC-Fr, IHC-P, WB
• Species Reactivity: Human

Product Description

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogen

Product Properties

• Clonality: Monoclonal
• Clone Designation: [5H9]
• Isotype: IgG1
• UniProt: P12830
• Buffer: Each vial contains 100 ul of 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Source: 5H9 is a Mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 H
• Formula: Each vial contains 100 ul of 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Application Notes

• Application Notes: 5H9 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin embedded tissues. For frozen tissues we recommend fixation with 1% formaldehyde in PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 for 10 minutes