The reactivity of the antiserum is restricted to salivary amylase. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against human saliva a single precipitin line is obtained and shows a reaction of full identity
Delipidated, heat inactivated, lyophilized, stable antiserum. No preservative added Total protein and IgG concentration are comparable to those of pooled normal sheep serum. No foreign proteins added Reconstitute the lyophilized antiserum by adding 1 ml
Source:
Human salivary amylase is an enzyme hydrolyzing the alpha-1,4-glucan links in polysaccharides. Its molecular weight is 55,000. The immunogen has been isolated from pooled saliva and purified to homogeneity for use in the antiserum production. Freunds co
Formula:
Delipidated, heat inactivated, lyophilized, stable antiserum. No preservative added Total protein and IgG concentration are comparable to those of pooled normal sheep serum. No foreign proteins added
Application Notes:
Precipitation assays. In immunoelectrophoresis in agarose-plates use 2 µl human saliva or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl plasma sa
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