Sheep anti Mouse IgG2a (subclass specific), Clone: [Polyclonal], Monoclonal
Biozol Catalog Number:
NMB-SHAM/IGG2A
Supplier Catalog Number:
ShAM/IgG2a
Alternative Catalog Number:
NMB-SHAM/IGG2A
Manufacturer:
NordicMubio
Host:
Sheep
Category:
Antikörper
The reactivity of the antiserum is directed to the subclass IgG2a. It reacts strongly withIg2a of the allelic types Igh-1a and Igh-1b, which include BALB/C, C57BL, CBA/J, SJ/J and SM/J. When used for the identification of IgG2a in other mouse strains the
Clonality:
Monoclonal
Clone Designation:
[Polyclonal]
Buffer:
Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. No foreign prote
Source:
Pools of purified homogenous IgG2a isolated from mouse serum of strains belonging to the allelic types Igh-1a and Igh-1b. Freunds complete adjuvant is used in the first step of the immunization procedure.
Formula:
Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. No foreign prote
Application Notes:
Precipitation assays. In immunoelectrophoresis use 2 µl or equivalent against 120 µl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and
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