A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg s
Concentration:
See vial for concentration
Buffer:
Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.
Formula:
Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.
Application Notes:
Suggested use: A volume of 10-25ul is suggested for immunobloting.
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