Total cell Iysates are useful as both positive and negative controls in immunoblotting. A431, a human epidermoid carcinoma cell line, and the EGF-stimulated A431 Iysates are used as negative and positive controls, respectively, when studying the phosphor
Concentration:
See vial for concentration
Buffer:
Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.
Formula:
Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.
Application Notes:
Optimal concentration should be evaluated by serial dilutions. Suggested use: A volume of 10-25ul is suggested for immunobloting.
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