Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Source:
Rabbits were immunized with a synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosie kinase 1
Purity:
Antigen Immunoaffiinity Purification
Formula:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Application Notes:
Antibody can be used for Western blotting (1-5µg/ml). Antibody shows some cross reactivity (~10% by ELISA) with other phosphoserine containing proteins. Optimal concentration should be evaluated by serial dilutions.
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