HLA-DPB1 Antibody (MHC II), Clone: [BraFB6], Mouse, Monoclonal

Product Overview

• Article Name: HLA-DPB1 Antibody (MHC II), Clone: [BraFB6], Mouse, Monoclonal
• Biozol Catalog Number: NSJ-V2578-20UG
• Supplier Catalog Number: V2578-20UG
• Alternative Catalog Number: NSJ-V2578-20UG
• Manufacturer: NSJ Bioreagents
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FACS, IF
• Species Reactivity: Human
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the HLA-DPB1 antibody.

Product Description

Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Clone Designation: [BraFB6]
• Isotype: Mouse IgG2b, kappa
• UniProt: P04440
• Buffer: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Form: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Target: HLA-DPB1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Application Notes: Optimal dilution of the HLA-DPB1 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.