TNF alpha Antibody, Clone: [J1D9], Mouse, Monoclonal

Product Overview

• Article Name: TNF alpha Antibody, Clone: [J1D9], Mouse, Monoclonal
• Biozol Catalog Number: NSJ-V2901-100UG
• Supplier Catalog Number: V2901-100UG
• Alternative Catalog Number: NSJ-V2901-100UG
• Manufacturer: NSJ Bioreagents
• Host: Mouse
• Category: Antikörper
• Application: FACS, IF, NeA
• Species Reactivity: Human
• Immunogen: Recombinant human protein was used as the immunogen for the TNF-alpha antibody.

Product Description

This antibody neutralizes TNF alpha biological activities. It prevents TNF alpha induced apoptosis in Jurkat cells. It also neutralizes HurTNFamediated cytotoxicity of L929 cells and inhibits tumor growth in mice. It protects mice against toxicity of Hur

Product Properties

• Clonality: Monoclonal
• Concentration: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Clone Designation: [J1D9]
• Isotype: Mouse IgG1, kappa
• UniProt: P01375
• Buffer: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Form: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Target: TNF alpha
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Inhibits tumor growth (order BSA/sodium azide-free format),Neutralizes rTNF- mediated cytotoxicity (order BSA/sodium azide-free format),Protects mice against toxicity of rhTNF- (order BSA/s
• Application Notes: Optimal dilution of the TNF-a antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.