VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit

Product Overview

• Article Name: VeriKine-HS Human Interferon Alpha All Subtype ELISA Kit
• Biozol Catalog Number: PBL-41115-1
• Supplier Catalog Number: 41115-1
• Alternative Catalog Number: PBL-41115-1
• Manufacturer: PBL Assay Science
• Category: Kits/Assays

Product Description

Quantitative determination of Hu-IFN alpha A, 2, A/D, B2, C, D, G, H, I, J1, K, 1, 4b and WA in serum, plasma and tissue culture media

Additional Information

Product Features: 

 

• Human IFN Alpha ELISA quantitates low level human interferon alpha in normal & autoimmune sera, EDTA plasma, and TCM with LLOQ 1.95 pg/ml
• Detects All 12 human IFN Alpha subtypes for accurate total IFN alpha measurement in samples
• Reproducible inter- (<10%) and intra-assay (< 8%) CVs in all tested matrices provide reliable data

 

Background: 

This high sensitivity Human IFN Alpha ELISA kit is designed to provide accurate low pg/ml measurement of ALL human interferon alpha subtypes in autoimmune sera, normal serum, EDTA plasma, and tissue culture media (TCM). This unique feature provides an improved view of global IFN-alpha production in sample analysis. 

Interferons (IFNs) are a family of mammalian cytokines initially characterized by their ability to inhibit viral infection. They are synthesized and secreted by most cell types in response to pathogens. In addition to their antiviral properties, IFNs have also been shown to exhibit anti-proliferative, immunomodulatory and many other activities.

In humans, IFN-α consists of a group of proteins that are greater than 85% homologous by amino acid sequence. Numerous individual human IFN-α subtypes have been identified; many display different properties. It remains unclear why there are multiple IFN-α subtypes. A variety of studies suggested they possess overlapping but also unique sets of biological activities.

 

Specifications

Compatibility: Serum, EDTA Plasma, Tissue Culture Media
ELISA Assay Range: 1.95 - 125 pg/ml
Speed: Typical Incubation time, 22 hours, 30 minutes
Specificity: Human Interferon Alpha
Cross-reacts with Cynomolgus/Rhesus IFN-α. No cross-reactivity against human IFN-β, IFN-γ, or IFN-ω; mouse or rat IFN-α, IFN-β or IFN-γ; or bovine IFN-τ.
Synonyms: IFN alpha, IFN-a, Multisubtype Interferon Alpha, Multisubtype Leukocyte Interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha

Inter-Assay CV: ≤ 10%
Intra-Assay CV: ≤ 8%
Spike Recovery: ≥ 80% in Serum

Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

 

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Materials Provided:

- Pre-coated microtiter plate(s)

- Plate Sealers

- Wash Solution Concentrate

- Human Interferon Alpha Standard, 10,000 pg/ml

- Sample Buffer

- Standard Diluent

- Antibody Concentrate

- HRP Conjugate Concentrate

- Antibody Diluent

- HRP Diluent

- TMB Substrate

- Stop Solution

 

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Representative Lower Limits of Quantitation and Lower Limits of Detection (pg/ml) in Normal Human Serum for each Human Interferon Alpha Subtype using PBL's High Sensitivity Human IFN Alpha ELISA (41115):

 

Product Properties

• Range: 1.95-125 pg/ml

Application Notes

• Application Notes: Assay Range: 1.95-125 pg/ml

Images

Global Human IFN Alpha-Specific Signals in Lupus Patient Sera using PBL's High Sensitivity Human IFN Alpha All Subtype ELISA (41115)

Lower Limit of Quantitation (LLOQ) and Lower Limit of Detection (LLOD) for 41115