VeriKine-HS Human Interferon Beta Serum ELISA Kit

Product Overview

• Article Name: VeriKine-HS Human Interferon Beta Serum ELISA Kit
• Biozol Catalog Number: PBL-41415-1
• Supplier Catalog Number: 41415-1
• Alternative Catalog Number: PBL-41415-1
• Manufacturer: PBL Assay Science
• Category: Kits/Assays

Product Description

Quantitative determination of Hu-IFN Beta in tissue culture media, serum and plasma

Additional Information

Product Features: 

• IFN-beta ELISA is designed to measure human interferon beta in serum, plasma, and tissue culture media with 1.2 pg/ml LLOQ sensitivity
• Suitable for measurement of IFN-beta in autoimmune serum (link)
• Suitable for measurement of trademarked interferon beta 1a and interferon beta 1b therapeutic molecules in human serum (link)
• ELISA assay provides > 90% serum spike-recovery
• Reproducible results with inter-assay CV < 8% and intra-assay CV < 10%
• No inhibition of IFN beta detection by 3-log excess of soluble IFNAR2 protein

 

Specifications

Compatibility: Serum, Plasma, Tissue Culture Media
ELISA Assay Range: 1.2 - 150 pg/ml
Speed: Incubation time, 3 hours, 30 minutes
Specificity: Human Interferon Beta 1a, Human Interferon Beta 1b (No cross-reactivity against human IFN-alpha, IFN-gamma, IFN-omega, IL-6; mouse IFN-alpha, IFN-beta; or rat IFN-beta.)
Synonyms: Human Beta Interferon, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B

 

Inter-Assay CV: < 8%
Intra-Assay CV: < 10%
Spike Recovery: > 90% in Serum

 

Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

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Materials Provided:

• Pre-coated microtiter plate
• Plate Sealers
• Wash Solution Concentrate
• Human Interferon Beta 1a Standard, 100,000 pg/ml
• Standard Diluent
• Sample Buffer
• Antibody Concentrate
• HRP Conjugate Concentrate
• Diluent Additive III
• Assay Diluent
• TMB Substrate
• Stop Solution

 

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Levels of Interferon Beta in 128 Normal Donor and 54 Multiple Sclerosis (MS) Patient Sera on & off IFN Beta therapies quantified using PBL's High Sensitivity Human IFN Beta ELISA (41415):

IFN Beta is quantifiable in 86% of MS patients on IFN Beta therapy, 4.1% of MS patients on other therapies and 1.5% of Normal Donor samples.

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Product Properties

• Range: 1.2-150 pg/ml

Application Notes

• Application Notes: Assay Range: 1.2-150 pg/ml