Dilute in PBS or medium which is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at 0.5-2ug/mL. Positive control: Jurkat cell lysate. Immunocytochemistry: use at 2ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 ug/mL.
Immunofl
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