Dilute in PBS or medium which is identical to that used in the assay system.
Application Notes:
Western Blot: Use at 1ug/ml. Positive control: Raji cell lysate Immunohistochemistry: use at 10ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Western blot analysis of PHAP expression in human Raji cell llysate with PHAP antibody at 1 ug/mL.
Immunohistochemistry of PHAP in human small intestine tissue with PHAP antibody at 10 ug/mL.
Immunofluorescence of PHAP in human small intestine tissue wit
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