Dilute in PBS or medium which is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at 0.25-1ug/mL. A band of ~60 kDa is detected. Immunohistochemistry: use at 5ug/mLFlow cytometry: use at 10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application.
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