Dilute in PBS or medium which is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at 1-5ug/mL. A band of ~90 kDa is detected. Immunoprecipitation: 5ug on 20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should determine optimal concentrations for their application.
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