Dilute in PBS or medium which is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at 0.5-1ug/mL. A band of ~52 kDa is detected. Immunohistochemistry: use at 4-5ug/mL Immunoprecipitation: 5ug on 10-20ul Protein A-Sepharose + 100ul sample. These are recommended concentrations. User should dete
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