Dilute in PBS or medium which is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at 1-5ug/mL. A band of ~69 kDa is detected.Immunohistochemistry / Immuno- fluorescence: use at 1-5 ug/mL. Immunoprecipitation: use 1-2ug per 100- 500ug total protein. These are recommended concentrations. User should d
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