Dilute in PBS or medium which is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at 0.2ug/mL (ECL). A band of ~70 kDa is detected. Immunoprecipitation: use at 1-2ug/mLImmunohistochemistry/Immunocyto- chemistry: use at 2 ug/mLUser should determine optimal concentrations for their applications. Posit
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