Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
ELISA: use at 1ug/ml with HO-1 on the solid phase. Immunoblotting: use at 0.5-1ug/ml. A band of ~32kDa is detected. Immunofluorescence: use at 5-10ug/ml Positive control: Human cell line lysates such as A431, A549, HeLa, HepG2, Ju
Immunoblotting: use at 0.5-1ug/ml. A band of ~32kDa is detected.
Immunofluorescence: use at 5-10ug/ml. Detection of HO-1 in formaldehyde-fixed HeLa cells.
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