Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at 1- 2ug/mL. A band of ~51kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Detection of Beclin 1 in 20ug 293T cell lysate.
Immunofluorescence: use at 10ug/ml. Detection of Beclin 1 in formaldehyde-fixed neuroblastoma SK
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