Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration) then dilute further for applications in PBS or media that is identical to that used in the assay system.
Application Notes:
Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50120. Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions. Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/
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