Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
Indirect ELISA: optimized for lead-RSA (rabbit serum albumin) on solid phase at 200ng/well. Ascites diluted 1:100,000 produces an O.D. signal of 0.5. Competition ELISA: with lead-RSA on the solid phase at 200ng/well, 50% inhibition of antibody bi
* VAT and and shipping costs not included. Errors and price changes excepted