Reconstitute the lyophilized antibody in 100ul of distilled water (final concentration 2.0mg/ml) for stock solution. Dilute the stock solution in 10mM PBS, pH 7.4 + 1.0% BSA immediately before use.
Application Notes:
Immunoblotting: use at 1-5ug/ml under reducing conditions. A band of ~100kDa is detected. These are recommended concentrations. End users should determine optimal concentrations for their applications.
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