Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
ELISA: 1:10,000-1:50,000 Western Blot: 1:500-1:30,000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250. Prepare working dilutions immediately before use. These are recommended dilutions. User shou
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