Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at a dilution of 1:10,000-1:200,000. Immunohistochemistry: use at a dilution of 1:250-1:2,500 Immunocytochemistry: use at a dilution of 1:100-1:500 ELISA: use at a dilution of 1:10,000-1:200,000. These
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