Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
ELISA: 1:20,000-1:200,000 Western Blot: 1:20,000-1:200,000 Immunohistochemistry: 1:500-1:5,000 Immunocytochemistry: 1:200-1:1,000. Prepare working dilutions immediately before use. These are recommended dilutions.
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