Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
ELISA Coating: 1:100-1:500, Primary: 1:1,000-1:30,000 Western Blot - Colorimetric 1:1,000-1:10,000, Chemiluminescent: 1:1,000-1:30,000 Immunohistochemistry: 1:200-1:2,000 Immuocytochemistry: 1:200-1:2,000 These are recomme
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