Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
ELISA: 1:10,000-1:100,000 Western Blot - Colorimetric: 1:1,000-1:10,000, Chemiluminescent: 1:1,000-1:30,000 Immunohistochemistry: 1:200-1:500 Immunocytochemistry: 1:200-1:500 These are recommended dilutions only. E
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