Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
Immunohistochemistry: use at a dilution of 1:150-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine
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