Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
ELISA: 1:500-1:5,000 Western Blot: 1:200-1:1,000 Immunohistochemistry: 1:200-1:500 Immunocytochemistry: 1:200-1:500 These are recommended dilutions only. End user should determine optimal dilutions for their applic
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