Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at a dilution of 1:20,000-1:400,000. Immunohistochemistry: use at a dilution of 1:500-1:5,000 Immunocytochemistry: use at a dilution of 1:200-1:1,000 ELISA: use at a dilution of 1:20,000-1:400,000. Thes
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