Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at a dilution of 1:2,000-1:20,000. Immunohistochemistry: use at a dilution of 1:200-1:2,000 Immunocytochemistry: use at a dilution of 1:200-1:2,000 ELISA: use at a dilution of 1:10,000-1:100,000. These
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