Dilute in PBS or medium that is identical to that used in the assay system.
Application Notes:
Immunoblotting: use at a dilution of 1:1,000-1:30,000. Immunohistochemistry: use at a dilution of 1:200-1:2,000 Immunocytochemistry: use at a dilution of 1:200-1:2,000 ELISA: for detection use at a dilution of 1:1,000-1:30,000, fo
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