This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino t
This antibody has been tested for use in ELISA and western blot. For western blot experiments, one can expect a band of ~110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate. A 1:500 dilution was effective for staining FFPE huma
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