Anti-Foxp3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Foxp3 conjugated to Keyhole Limpet Hemocyanin (KLH).
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000-1:50,000, Flow Cytometry: 1:25, IF Microscopy: 15µg/mL, WB: 1:1000
Application Notes:
Anti-Foxp3 Antibody has been tested in ELISA, WB, IF, and FLOW. Expect a band for isoforms 1 and 4 at ~47.2 and 44.4kDa in western blot using appropriate tissues and lysates. Positive control used: NIH/3T3 cells in Immunofluorescence and Flow cytometry.
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