0.01 M Tris Cl, 0.15 M Sodium Chloride, 0.001 M EDTA, pH 7.4
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: 1:5,000 - 1:25,000, Gel Shift: See protocol, WB: 1:500 - 1:3,000
Application Notes:
Gel Shift/Gel Super Shift Assays: the electrophoretic gel shift assay is used to detect sequence specific DNA-binding proteins present in nuclear extracts. For NFkB generally a HeLa nuclear extract is used. In the assay, a consensus oligonucleotide is en
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