Protein A Alkaline Phosphatase Conjugated, AP

Product Overview

• Article Name: Protein A Alkaline Phosphatase Conjugated, AP
• Biozol Catalog Number: ROC-PA00-05
• Supplier Catalog Number: PA00-05
• Alternative Catalog Number: ROC-PA00-05
• Manufacturer: Rockland Immunochemicals
• Category: Proteine/Peptide
• Application: DOT, ELISA
• Conjugation: AP
• Alternative Names: ProA, Staphylococcus A protein, ALP, Protein A Alk Phos

Product Properties

• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 38507
• UniProt: P38507
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: ELISA: 1:8,000 - 1:32,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Protein A Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that ca