Rabbit IgG-UNLB, Polyclonal

Product Overview

• Article Name: Rabbit IgG-UNLB, Polyclonal
• Biozol Catalog Number: SBA-0111-01
• Supplier Catalog Number: 0111-01
• Alternative Catalog Number: SBA-0111-01
• Manufacturer: SouthernBiotech
• Host: Rabbit
• Category: Antikörper
• Application: AM, Blocking, ChIP, Control, ELISA, FC, ICC, IHC, IHC-Fr, IHC-P, IP, WB
• Conjugation: Unconjugated

Product Description

Purified Rabbit IgG for use as a control in flow cytometry, immunocytochemistry / immunocytochemistry, western blotting, immunoprecipitation, ChIP, microarray, and in vitro assays and as a standard in ELISA.

Product Properties

• Clonality: Polyclonal
• Isotype: IgG
• Form: Purified
• Antibody Type: Secondary Antibody

Images

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.

Lane 1 - 1 mg Rabbit IgG Lane 2 - 0.5 mg Rabbit IgG Rabbit IgG (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit IgG-AP (SB Cat. No. 4090-04). Proteins were visualized using BCIP/NBT One Component Membrane Substrate (Blue), Solution (SB Cat. No. 0430-01).

ELISA plate was coated with serially diluted Rabbit IgG-UNLB (SB Cat. No. 0111-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.

Tissue lysate from rTgFKBP5 double transgenic mouse hippocampus was pre-cleared with Mouse IgG-UNLB (SB Cat. No. 0107-01) and immunoprecipitated with anti-GluR1 or Rabbit IgG-UNLB (SB Cat. No. 0111-01). Protein was resolved by electrophoresis, transferred to membrane, and probed with anti-GluR1 or anti-HSP90 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05), an HRP conjugated biotin binding protein, and chemiluminescent detection. Image from Blair LJ, Criado-Marrero M, Zheng D, Wang X, Kamath S, Nordhues BA, et al. The disease-associated chaperone FKBP51 impairs cognitive function by accelerating AMPA receptor recycling. eNeuro. 2019;6:e0242-18.2019. Figure 8(c)

Frozen mouse spinal cord sections were stained with anti-BAFF-R (right) and Rabbit IgG-UNLB (SB Cat No. 0111-01; left) followed by a CY5 conjugated secondary antibody. Images from Tada S, Yasui T, Nakatsuji Y, Okuno T, Koda T, Mochizuki H, et al. BAFF controls neural cell survival through BAFF receptor. PLoS One. 2013;8(7):e70924. Figure 1(c/d)

Human T cell leukemia cell line Jurkat was stained with Rabbit IgG-UNLB (SB Cat. No. 0111-01; gray) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01) followed by Mouse Anti-Rabbit IgG-PE (SB Cat. No. 4090-09).

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05) followed by chemiluminescent detection.

Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) followed by Streptavidin-HRP (SB Cat. No. 7105-05) and chemiluminescent detection.