Goat Anti-Mouse IgG1-HRP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.
Clonality:
Polyclonal
Isotype:
IgG
Form:
Purified
Antibody Type:
Secondary Antibody
ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG1, Human ads-HRP (SB Cat. No. 1070-05).
Lane 1 - DEC Monoclonal Antibody
Lane 2 - DEC-HER2 Monoclonal Antibody
Lane 3 - Control Ig-HER2 Monoclonal Antibody
HER2 extracellular domain was cloned in frame into the COOH terminus of anti-DEC (DEC-HER2). DEC, DEC-HER2, and control mouse IgG were resolved by electrophoresis, transferred to membrane, and probed with Goat Anti-Mouse IgG1, Human ads-HRP (SB Cat. No. 1070-05) and chemiluminescent detection.
Image from Wang B, Zaidi N, He L, Zhang L, Kuroiwa JM, Keler T, et al. Targeting of the non-mutated tumor antigen HER2/neu to mature dendritic cells induces an integrated immune response that protects against breast cancer in mice. Breast Cancer Res. 2012;14:R39. Figure 1(b)
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