Goat Anti-Mouse IgG2a-Biotin antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.
Clonality:
Polyclonal
Isotype:
IgG
Form:
Purified
Antibody Type:
Secondary Antibody
ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
TGFB1 stimulated human dermal fibroblasts were stained with anti-ACTA2 followed by Goat Anti-Mouse IgG2a, Human ads-BIOT (SB Cat. No. 1080-08), CY3 conjugated Streptavidin, and DAPI.
Image from Ploeger DT, Hosper NA, Schipper M, Koerts JA, de Rond S, Bank RA. Cell plasticity in wound healing: paracrine factors of M1/ M2 polarized macrophages influence the phenotypical state of dermal fibroblasts. Cell Commun Signal. 2013;11:29. Figure 5(b)
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