Goat Anti-Mouse IgG2b, Human ads-HRP, Polyclonal

Product Overview

• Article Name: Goat Anti-Mouse IgG2b, Human ads-HRP, Polyclonal
• Biozol Catalog Number: SBA-1090-05
• Supplier Catalog Number: 1090-05
• Alternative Catalog Number: SBA-1090-05
• Manufacturer: SouthernBiotech
• Host: Goat
• Category: Antikörper
• Application: ELISA, ELISpot, ICC, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse
• Conjugation: HRP

Product Description

Goat Anti-Mouse IgG2b-HRP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.

Product Properties

• Clonality: Polyclonal
• Isotype: IgG
• Form: Purified
• Antibody Type: Secondary Antibody

Images

ELISA plate was coated with purified mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05).

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human MMP-1-UNLB (SB Cat. No. 12011-01) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 8-UNLB (SB Cat. No. 10080-01; IgG2a) and Mouse Anti-Human MMP-1-UNLB (SB Cat. No. 12011-01; IgG2b) followed by Goat Anti-Mouse IgG2a, Human ads-AP (SB Cat. No. 1080-04) and Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), Red AP, DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Phospho-Histone H3 (Thr3)-UNLB (SB Cat. No. 13500-01; right) followed by Goat Anti-Mouse IgG2b, Human ads-HRP (SB Cat. No. 1090-05), DAB, and mounted with Fluoromount-G® (SB Cat. No. 0100-01).

Lane 1 - Untreated Jurkat whole cell lysate Lane 2 - TSA-treated Jurkat whole cell lysate (400 nM, 12 hr) Cell lysates were resolved by electrophoresis, transferred to PVDF membrane, probed with Mouse Anti-Acetyl-Histone H3 (Lys4)-UNLB (SB Cat. No. 13600-01), and visualized using Goat Anti-Mouse IgG2b, Human SP ads-HRP (SB Cat. No. 1090-05) secondary antibody and chemiluminescent detection.