Goat Anti-Rat Ig-HRP antibody with minimal reactivity to mouse proteins for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.
Clonality:
Polyclonal
Isotype:
IgG
Form:
Purified
Antibody Type:
Secondary Antibody
Lysates from mouse embryonic fibroblasts expressing no Bak (Bax-/-Bak-/- (DKO)), mouse Bak (Bax-/-), or WT human Bak (in DKO) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with anti-Bak followed by Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05) and chemiluminescent detection.
Image from Alsop AE, Fennell SC, Bartolo RC, Tan IK, Dewson G, Kluck RM. Dissociation of Bak α1 helix from the core and latch domains is required for apoptosis. Nat Commun. 2015;6:6841. Figure 1(a)
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